解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Suberoylanilide hydroxamic acid (SAHA) is a potent histone deacetylase (HDAC) inhibitor. Inhibitors of HDACs are used in cancer therapy based on the role HDACs play in transcription by regulating chromatin compaction and non-histone proteins such as transcription factors. Profiling of HDAC expression is of interest in...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000717
更新日期:2011-10-01 00:00:00
abstract::Methyl parathion (MP) is a widely used organophosphorus pesticide, which has been related to a broad spectrum of toxic effects on environmental organisms. The present study investigated the changes in the protein profile of enriched membrane fraction from zebrafish (Danio rerio) brain exposed to three concentrations (...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100084
更新日期:2011-09-01 00:00:00
abstract::It becomes increasingly clear that separation of pure cell populations provides a uniquely sensitive and accurate approach to protein profiling in biological systems and opens up a new area for proteomic analysis. The method we described could simultaneously isolate population of hepatocytes (HCs), hepatic stellate ce...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100157
更新日期:2011-09-01 00:00:00
abstract::Sea star coelomic fluid is in contact with all internal organs, carrying signaling molecules and a large population of circulating cells, the coelomocytes. These cells, also known as echinoderm blood cells, are responsible for the innate immune responses and are also known to have an important role in the first stage ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000745
更新日期:2011-09-01 00:00:00
abstract::Two-dimensional difference gel electrophoresis (2-D DIGE) allows for reliable quantification of global protein abundance changes. The threshold of significance for protein abundance changes depends on the experimental variation (biological and technical). This study estimates biological, technical and total variation ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201100071
更新日期:2011-08-01 00:00:00
abstract::Serum depletion strategies are commonly implemented in order to remove abundant proteins, increasing the number of proteins detected in a biomarker study. The IgY spin columns used in this study bind 12 and 14 primate proteins, respectively. 1-D SDS-PAGE and 2-DE revealed a suboptimal performance of the IgY spin colum...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000587
更新日期:2011-08-01 00:00:00
abstract::Immune proteomics is an increasingly powerful tool for the investigation of the adaptive immune response to natural encounters between micro-organisms and their hosts. The versatile species Staphylococcus aureus serves to illustrate how these techniques can be employed to appreciate the complexity and diversity of the...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100010
更新日期:2011-08-01 00:00:00
abstract::Corynebacteria belong to the high G+C Gram-positive bacteria (Actinobacteria) and are closely related to Mycobacterium and Nocardia species. The best investigated member of this group of almost seventy species is Corynebacterium glutamicum, a soil bacterium isolated in 1957, which is used for the industrial production...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201000786
更新日期:2011-08-01 00:00:00
abstract::Antibiotics disturb the physiological homeostasis of bacterial cells by interfering with essential cellular functions or structures. The bacterial proteome adjusts quickly in response to antibiotic challenge. This physiological response is specifically tailored to overcome the inflicted damage and, thus, closely linke...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201100046
更新日期:2011-08-01 00:00:00
abstract::Due to the lack of precise markers indicative of its occurrence and progression, coronary artery disease (CAD), the most common type of heart diseases, is currently associated with high mortality in the United States. To systemically identify novel protein biomarkers associated with CAD progression for early diagnosis...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000202
更新日期:2011-07-01 00:00:00
abstract::Most MS-based glycomic and glycoproteomic analyses focus on identifying changes in terminal glyco-epitopes represented by sialylation and fucosylation at specific positions of the terminal N-acetyllactosamine units. Much less attention was accorded to the underlying linear or branched poly-N-acetyllactosamine extensio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000794
更新日期:2011-07-01 00:00:00
abstract::Basal-like breast cancers are commonly negative for expression of estrogen and progesterone receptors and HER-2 (triple-negative breast cancer), which makes this subtype of breast cancers more aggressive and less responsive to standard treatment. We have applied a small-scale chemical proteomics method using bisindoly...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000801
更新日期:2011-07-01 00:00:00
abstract::Affinity purification coupled to mass spectrometry (AP-MS) is gaining widespread use for the identification of protein-protein interactions. It is unclear, however, whether typical AP sample complexity is limiting for the identification of all protein components using standard one-dimensional LC-MS/MS. Multidimensiona...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000571
更新日期:2011-07-01 00:00:00
abstract::Proteases are critical in many physiological processes and the human genome encodes for 566 predicted proteolytic enzymes. Therefore, there is great interest in identifying and characterizing physiologic protease-substrate relationships. The coagulation cascade is a well-described network of serine proteases. However,...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000674
更新日期:2011-06-01 00:00:00
abstract::The array of biomolecules generated by a functioning ecosystem represents both a potential resource for sustainable harvest and a potential indicator of ecosystem health and function. The cupped leaves of the carnivorous pitcher plant, Sarracenia purpurea, harbor a dynamic food web of aquatic invertebrates in a fully ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000256
更新日期:2011-06-01 00:00:00
abstract::CID has become a routine method for fragmentation of peptides in shotgun proteomics, whereas electron transfer dissociation (ETD) has been described as a preferred method for peptides carrying labile PTMs. Though both of these fragmentation techniques have their obvious advantages, they also have their own drawbacks. ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000547
更新日期:2011-06-01 00:00:00
abstract::Serum is an ideal biological sample that contains an archive of information due to the presence of a variety of proteins released by diseased tissue, and serum proteomics has gained considerable interest for the disease biomarker discovery. Easy accessibility and rapid protein changes in response to disease pathogenes...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201000460
更新日期:2011-06-01 00:00:00
abstract::Menthol is a naturally occurring cyclic monoterpene used in oral hygiene products, confectionary, pharmaceuticals, cosmetics, pesticides, and as a flavoring agent. In the present study, we analyzed the differentially expressing proteome in L-menthol-treated Caco-2 cell line as it was found to inhibit cell proliferatio...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000691
更新日期:2011-05-01 00:00:00
abstract::Rice is susceptible to cold stress and with a future of climatic instability we will be unable to produce enough rice to satisfy increasing demand. A thorough understanding of the molecular responses to thermal stress is imperative for engineering cultivars, which have greater resistance to low temperature stress. In ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000727
更新日期:2011-05-01 00:00:00
abstract::Proteomics is increasingly being used to understand enzyme expression and regulatory mechanisms involved in the accumulation of storage reserves in crops with sequenced genomes. During the past six years, considerable progress has been made to characterize proteomes of both mature and developing seeds, particularly oi...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201000644
更新日期:2011-05-01 00:00:00
abstract::Aphids are major insect pests of cereal crops, acting as virus vectors as well as causing direct damage. The responses of wheat to infestation by cereal aphid (Sitobion avenae) were investigated in a proteomic analysis. Approximately, 500 protein spots were reproducibly detected in the extracts from leaves of wheat se...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.200900801
更新日期:2011-05-01 00:00:00
abstract::Adaptation of skeletal muscle to repeated bouts of endurance exercise increases aerobic capacity and improves mitochondrial function. However, the adaptation of human skeletal muscle mitochondrial proteome to short-term endurance exercise training has not been investigated. Eight sedentary males cycled for 60 min at 8...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000597
更新日期:2011-04-01 00:00:00
abstract::The computational simulation of complete proteomic data sets and their utility to validate detection and interpretation algorithms, to aid in the design of experiments and to assess protein and peptide false discovery rates is presented. The simulation software has been developed for emulating data originating from da...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000576
更新日期:2011-03-01 00:00:00
abstract::Topoisomerase (topo) II catalyzes topological changes in DNA. Although both human isozymes, topo IIα and β are phosphorylated, site-specific phosphorylation of topo IIβ is poorly characterized. Using LC-MS/MS analysis of topo IIβ, cleaved with trypsin, Arg C or cyanogen bromide (CNBr) plus trypsin, we detected four +8...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000194
更新日期:2011-03-01 00:00:00
abstract::Immuno-precipitation (IP) experiments using MS provide a sensitive and accurate way of characterising protein complexes and their response to regulatory mechanisms. Differences in stoichiometry can be determined as well as the reliable identification of specific binding partners. The quality control of IP and protein ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000548
更新日期:2011-03-01 00:00:00
abstract::In recent years, standardization and quality control have become important key points in industry, e.g. in drug discovery and for developing medical products. Is quality control in academic Proteomics a minor problem nowadays, where standard data formats and public repositories for data sharing exist? In this article,...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000441
更新日期:2011-03-01 00:00:00
abstract::Reporter ion-based methods are among the major techniques to quantify peptides and proteins. Two main labels, tandem mass tag (TMT) and iTRAQ, are widely used by the proteomics community. They are, however, often applied as out-of-the-box methods, without thorough quality control. Thus, due to undiscovered limitations...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000711
更新日期:2011-03-01 00:00:00
abstract::In this study, we performed the first high-throughput and comprehensive proteomic profiling of the rat hippocampal proteome. Using a combination of 2-D LC-MS and data analysis with the Rosetta Elucidator(®) system, we identified 1340 unique proteins. Functional classification showed that most of these were associated ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000525
更新日期:2011-02-01 00:00:00
abstract::Trichodesmium is a marine filamentous diazotrophic cyanobacterium and an important contributor of "new" nitrogen in the oligotrophic surface waters of the tropical and sub-tropical oceans. It is unique in that it exclusively fixes N(2) at daytime, although it belongs to the non-heterocystous filamentous segment of the...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000382
更新日期:2011-02-01 00:00:00
abstract::Phosphorylation of proteins is one of the most prominent PTMs and for instance a key regulator of signal transduction. In order to improve our understanding of cellular phosphorylation events, considerable effort has been devoted to improving the analysis of phosphorylation by MS-based proteomics. Different enrichment...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201000489
更新日期:2011-02-01 00:00:00
abstract::Mass spectrometry-based proteomics is used to gain insight into the abundance and subcellular localization of cellular signaling components, the composition of molecular complexes and the regulation of signaling pathways. Multicellular organisms have evolved signaling networks and fast responses to stimuli that can be...
journal_title:Proteomics
pub_type: 杂志文章,评审
doi:10.1002/pmic.201000418
更新日期:2011-02-01 00:00:00
abstract::Absolute protein concentration determination is becoming increasingly important in a number of fields including diagnostics, biomarker discovery and systems biology modeling. The recently introduced quantification concatamer methodology provides a novel approach to performing such determinations, and it has been appli...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000454
更新日期:2011-01-01 00:00:00
abstract::Diurnal physiological acclimation regulated by a circadian system is an advantage for plant fitness. The circadian system is composed of a signal input, the clock and output pathways. Understanding the regulation mechanism of the output pathways remains a major challenge. Diurnal proteomic change reflects the state of...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000065
更新日期:2011-01-01 00:00:00
abstract::Large-scale interaction studies contribute the largest fraction of protein interactions information in databases. However, co-purification of non-specific or indirect ligands, often results in data sets that are affected by a considerable number of false positives. For the fraction of interactions mediated by short li...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000030
更新日期:2011-01-01 00:00:00
abstract::This review is a meta-analysis of data describing proteins regulated by morphine influence studied worldwide across last years administration. Up to date (July 2010), 15 studies concerning this subject have been published. Animal models, examined brain structures, the route of morphine administration and proteomic pla...
journal_title:Proteomics
pub_type: 杂志文章,meta分析,评审
doi:10.1002/pmic.200900848
更新日期:2011-01-01 00:00:00
abstract::Spatial organisation of proteins according to their function plays an important role in the specificity of their molecular interactions. Emerging proteomics methods seek to assign proteins to sub-cellular locations by partial separation of organelles and computational analysis of protein abundance distributions among ...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000359
更新日期:2010-12-01 00:00:00
abstract::Identification of differential proteomic responses to cadmium (Cd) would provide a means for better understanding the survival mechanisms of the earthworm, Eisenia fetida, living in a Cd-polluted environment, and the stress responses can be assessed by ecotoxiptoromics approaches. Extracts of whole earthworm collected...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000209
更新日期:2010-12-01 00:00:00
abstract::The allergen content of standardized pollen material is crucial for an effective diagnosis and treatment. However, variations in IgE reactivities of allergic patients to different preparations of Phleum pratense pollen have been reported. In order to define and directly compare the allergen composition of pollen prepa...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000451
更新日期:2010-12-01 00:00:00
abstract::The Asia Oceania Human Proteome Organisation (AOHUPO) has embarked on a Membrane Proteomics Initiative with goals of systematic comparison of strategies for analysis of membrane proteomes and discovery of membrane proteins. This multilaboratory project is based on the analysis of a subcellular fraction from mouse live...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000126
更新日期:2010-11-01 00:00:00
abstract::Identification of the phosphorylated residues of bacterial Ser/Thr protein kinase (STPK) substrates still represents a challenging task. Herein, we present a new strategy allowing the rapid determination of phosphoacceptors in kinase substrates, essentially based on the dual expression of the kinase with its substrate...
journal_title:Proteomics
pub_type: 杂志文章
doi:10.1002/pmic.201000316
更新日期:2010-11-01 00:00:00